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quickchange ii method (Agilent technologies)

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Agilent technologies quickchange ii method
Quickchange Ii Method, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quickchange+ii+method/pmc10382558-208-13-16
Average 90 stars, based on 1 article reviews

quickchange ii method - by Bioz Stars, 2026-09

90/100 stars

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Construct:

Article Title: Glycine acylation and trafficking of a new class of bacterial lipoprotein by a composite secretion system
Article Snippet: .. Point mutations were constructed using the QuickChange II method (Aglient). ..

Article Title: Distinct Phosphorylation Clusters Determine the Signaling Outcome of Free Fatty Acid Receptor 4/G Protein-Coupled Receptor 120.
Article Snippet: .. This construct was used as template to generate various mutants (see Results) using the Quickchange II method (Stratagene, UK). ..

Sequencing:

Article Title: Subunit gating resulting from individual protonation events in Kir2 channels
Article Snippet: .. Point mutations in both chicken pCDNA3.1-Kir2.2 and human pCDNA3.1-Kir2.1 were introduced using the QuickChange II method (Agilent Technologies) with the entire coding region verified by sequencing. ..

Mutagenesis:

Article Title: Azurin as a protein scaffold for a low-coordinate non-heme iron site with a small-molecule binding pocket
Article Snippet: .. The Met121Ala mutation was accomplished according to the QuickChange II method (Stratagene, La Jolla, CA) using the following primer in which the mutated site is underlined. .. 5′-GGGTCACTCCGCACTG GCG AAAGGTACCCTGACTC-3′ The parental DNA was digested with Dpn1 at 37 °C for 2 h and the final product was transformed into XL1-Blue supercompetent cells (Stratagene, La Jolla, CA).